Journal: Cellular and Molecular Immunology

Article Title: Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells

doi: 10.1038/cmi.2016.63

Figure Lengend Snippet: IL-33 expression in acute experimental and clinical liver fibrosis. (a) The hepatic IL-33 expression following bile-duct ligation (BDL) was assessed in C57BL/6 mice at several time points by real-time PCR and western blot, and (b) IL-33 serum levels were determined by ELISA. Mean values±s.e.m. are given of two independent studies with groups of eight mice (*P<0.05, **P <0.01, ***P <0.001). (c) IL-33 in human liver tissue homogenates (ELISA) after partial hepatectomy of early-stage hepatocellular carcinoma with fibrosis or controls with hepatic hemangioma. The data are expressed as the median and range (0, 25, 50, 75 and 100%) from 24 fibrotic and 20 normal liver tissues (***P <0.001). (d) Cellular expression of IL-33 in fibrotic human livers was identified by immunofluorescence (IF) using FITC-labeled anti huIL-33 antibody, PE-labeled CK-19 (cholangiocytes), PE-labeled GFAP (HSC) or PE-labeled albumin (hepatocytes). Nuclei were counterstained with DAPI (magnification × 400, scale bar, 50 μm).

Article Snippet: In preliminary trials, mice were given an intraperitoneal injection of recombinant mouse IL-33 (1, 5 or 10 μg per day, R&D Systems, Abingdon, UK) 3 days before BDL, and 5 μg was finally used in the experiments.

Techniques: Expressing, Ligation, Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Labeling

Journal: Cellular and Molecular Immunology

Article Title: Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells

doi: 10.1038/cmi.2016.63

Figure Lengend Snippet: BDL-induced liver injury and fibrosis are reduced in the absence of ST2. Liver injury following bile-duct ligation (BDL) was analyzed in C57BL/6 and ST2-deficient (KO) mice. (a) Macro- and microphotographs demonstrate attenuated liver inflammation, necrosis and fibrosis in ST2-KO mice (H&E and Masson staining, original magnification × 200). (b) Serum alanine aminotransferase and aspartate aminotransferase at 0, 1, 3, 10 and 21 days after BDL. (c) Inflammation was assessed in liver homogenates of C57BL/6 and IL-33/ST2-KO mice at 0, 1, 3, 10 and 21 days after BDL. IL-1β, KC and thymic stromal lymphopoietin were reduced in ST2-KO mice compared with C57BL/6 mice. (d) Hepatic collagen expression was assessed with a Sircol assay and real-time PCR for collagen 1a1. The data are expressed as median values±s.e.m. (n=6–8 mice/group) and are representative of three independent experiments (*P<0.05, **P<0.01, ***P<0.001).

Article Snippet: In preliminary trials, mice were given an intraperitoneal injection of recombinant mouse IL-33 (1, 5 or 10 μg per day, R&D Systems, Abingdon, UK) 3 days before BDL, and 5 μg was finally used in the experiments.

Techniques: Ligation, Staining, Expressing, Real-time Polymerase Chain Reaction

Journal: Cellular and Molecular Immunology

Article Title: Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells

doi: 10.1038/cmi.2016.63

Figure Lengend Snippet: Recombinant IL-33 exacerbates inflammation in C57BL/6 mice. Recombinant mouse IL-33 was injected 3 days before BDL (intraperitoneally at 5 μg per day) into C57BL/6 mice. (a) Serum alanine aminotransferase and aspartate aminotransferase levels from WT BL6 mice without or with rmIL-33 (0 μg, 1 μg, 5 μg and 10 μg) at 1 day. The data are expressed as the mean values±s.e.m. (n=6–8 mice/group; (a) values are significantly different from the BL6 sham group; (b) not significantly different from the 5 μg IL-33 group). (b and c) Increased liver injury and inflammation, as assessed by hematoxylin and eosin staining, myeloperoxidase and Suzuki score. (d) Hepatic IL-1β, thymic stromal lymphopoietin and granulocyte-macrophage colony stimulating factor were increased. The data are expressed as the mean values±s.e.m. (n=6–8 mice/group) and are representative of three independent experiments (*P<0.05; **P<0.01; ns, not significant).

Article Snippet: In preliminary trials, mice were given an intraperitoneal injection of recombinant mouse IL-33 (1, 5 or 10 μg per day, R&D Systems, Abingdon, UK) 3 days before BDL, and 5 μg was finally used in the experiments.

Techniques: Recombinant, Injection, Staining

Journal: Cellular and Molecular Immunology

Article Title: Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells

doi: 10.1038/cmi.2016.63

Figure Lengend Snippet: IL-33 activates hepatic stellate cells (HSCs) via mitogen-activated protein kinases signaling. Mouse HSCs were isolated from naive C57BL/6 and ST2-KO mice and activated with rmuIL-33 in vitro (0, 1, 10, 50 or 100 ng/ml). IL-6, TGF-β, α-SMA RNA transcription and soluble collagen in the supernatant were increased at 24 h (a–d). HSCs expressed increased phosphorylated JNK, ERK or p38 on activation by rmuIL-33 at 24 h, which was ST2-dependent. Levels of JNK, ERK or p38 phosphorylation were quantified by ImageJ software (e). The JNK inhibitor SP600125, ERK/MEK1 inhibitor PD98059, or p38 inhibitor SB203580 were given 1 h before rmIL-33 at 100 ng/ml and inhibited collagen production, as measured by Sircol assay (f). Mean values±s.e.m. of a representative study of three independent experiments are shown; comparisons between subgroups were performed by one-way ANOVA followed by a Newman–Keuls posttest: *P<0.05, **P<0.01, ***P<0.001 (compared with cells cultured in medium).

Article Snippet: In preliminary trials, mice were given an intraperitoneal injection of recombinant mouse IL-33 (1, 5 or 10 μg per day, R&D Systems, Abingdon, UK) 3 days before BDL, and 5 μg was finally used in the experiments.

Techniques: Isolation, In Vitro, Activation Assay, Software, Cell Culture

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway

doi: 10.1590/ACB360501

Figure Lengend Snippet: Survival rate of mice in sham operation, model, IL-33 -/- , and IL-33 -/- intervention groups. Survival rate of mice in sham operation, model, IL-33 -/- , andIL-33 -/- intervention groups (n = 7). Survival rate of mice in sham operation, model, IL-33 -/- , and IL-33 -/- intervention groups (n = 7); && p < 0.01 vs. sham operation group; ^^ p < 0.01 vs. model group; ## p < 0.01 vs. IL-33 -/- group.

Article Snippet: After operation, recombinant murine IL-33 (TransGen Biotech™, Beijing, China) was intraperitoneally injected into the mice at 0.5 mg/kg in the IL-33 -/- intervention group, while the same volume of normal saline was injected into the mice in the model group, the sham operation group, and the IL-33 -/- group.

Techniques:

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway

doi: 10.1590/ACB360501

Figure Lengend Snippet: Macrophage identification and mRNA expression level of IL-33 in mice peritoneal lavage fluid. (a) Immunofluorescence to detect CD11b expression in peritoneal lavage fluid; (b) mRNA expression level of IL-33 in sham operation, model,IL-33 -/- , and IL-33 -/- intervention groups (n = 5); && p < 0.01 vs. sham operation group; ^^ p < 0.01 vs. model group; ## p < 0.01 vs. IL-33 -/- group.

Article Snippet: After operation, recombinant murine IL-33 (TransGen Biotech™, Beijing, China) was intraperitoneally injected into the mice at 0.5 mg/kg in the IL-33 -/- intervention group, while the same volume of normal saline was injected into the mice in the model group, the sham operation group, and the IL-33 -/- group.

Techniques: Expressing, Immunofluorescence

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway

doi: 10.1590/ACB360501

Figure Lengend Snippet: Content of inflammatory factors detected via ELISA. (a) Content of IL-33; (b) Content of IL-1β; (c) content of IL-18. Content of IL-33,IL-1β, and IL-18 in the serum of mice in sham operation, model, IL-33 -/- , and IL-33 -/- intervention groups (n = 5); && p < 0.01 vs. sham operation group; ^^ p < 0.01 vs. model group; ## p < 0.01 vs. IL-33 -/- group.

Article Snippet: After operation, recombinant murine IL-33 (TransGen Biotech™, Beijing, China) was intraperitoneally injected into the mice at 0.5 mg/kg in the IL-33 -/- intervention group, while the same volume of normal saline was injected into the mice in the model group, the sham operation group, and the IL-33 -/- group.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway

doi: 10.1590/ACB360501

Figure Lengend Snippet: Expression level of pyroptosis-related proteins in macrophages of mice. (a) Bands; (b) statistical graph. The expression level of pro-caspase-1 p10 in macrophages of mice in sham operation, model, IL-33 -/- ,and IL-33 -/- intervention groups (n = 5); && p < 0.01 vs. sham operation group; ^^ p < 0.01 vs. model group; ## p < 0.01 vs. IL-33 -/- group.

Article Snippet: After operation, recombinant murine IL-33 (TransGen Biotech™, Beijing, China) was intraperitoneally injected into the mice at 0.5 mg/kg in the IL-33 -/- intervention group, while the same volume of normal saline was injected into the mice in the model group, the sham operation group, and the IL-33 -/- group.

Techniques: Expressing

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway

doi: 10.1590/ACB360501

Figure Lengend Snippet: Expression level of NF-κB/p38 MAPK signaling pathway-related proteins in macrophages of mice determined by means of Western blotting. (a) Bands; (b) statistical graph of IκB-α; (c) statistical graph of NF-κB p65; (d) statistical graph of p-p38 MAPK. The expression levels of IκB-α and NF-κB p65 proteins of mice in sham operation, model, IL-33 -/- , and IL-33 -/- intervention groups (n = 5); && p < 0.01 vs. sham operation group; ^^ p < 0.01 vs. model group; ## p < 0.01 vs. IL-33 -/- group.

Article Snippet: After operation, recombinant murine IL-33 (TransGen Biotech™, Beijing, China) was intraperitoneally injected into the mice at 0.5 mg/kg in the IL-33 -/- intervention group, while the same volume of normal saline was injected into the mice in the model group, the sham operation group, and the IL-33 -/- group.

Techniques: Expressing, Western Blot